G. Karthikeyan, S. Rajaram, S. Arulraja


Diabetes mellitus is a rapidly increasing incidence in the developing and developed countries throughout the world. The present investigation was performed to assess the anti-diabetic activity of Nymphaea alba flower extract against streptozotocin (STZ) induced diabetic condition in rat model. N.alba flower extract was orally given to experimentally induced diabetic animals (at the concentration of 200, 400 and 800 mg/kg body weight). Treatment of N.alba flower extract significantly (p < 0.05) lowered the level of blood glucose, serum insulin and HbA1C levels in experimentally induced diabetic animals in a concentration dependent fashion. A significant (p < 0.05) reduction in the levels of antioxidant enzymes such as glutathione peroxiodase, glutathione S transferase, catalase and superoxide dismutase were observed in experimental diabetic animal model. Upon treatment with N.alba flower extract the above said antioxidant enzyme levels were reverted back to normalcy in a dose dependent fashion. The present investigation revealed that N.alba flower extract exerted strongly antidiabetic action by restoring the antioxidant system and anti-hyperglycemic activity. Consequently, it might be used as a safer harmonizing substance in the diabetic conditions.


Full Text:



Golbidi S, Badran M, Laher I. Antioxidant and Anti-Inflammatory Effects of Exercise in Diabetic Patients. Exp Diabetes Res 2012; 2012: 941868.

Montonen J, Knekt P, Jarvinen R, Reunanen A. Dietary Antioxidant Intake and Risk of Type 2 Diabetes. Diabetes Care 2004; 27:362-6.

Abu-Zaida ME, Mashaly IA, Abd El-Monem M, Torky M. Economic potentials of some aquatic plants growing in North East Nile delta, Egypt. J Applied Sci. 2008; 8(1):1395-1405.

Ebaid, B. Abdel-Salam, I. Hassan, J. Al-Tamimi, A. Metwalli, and I. Alhazza, “Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response,” Lipids in Health and Disease, 2015; 14 (132) DOI: 10.1186/s12944-015-0136-9).

Trinder, P. Determination of glucose in blood using glucose oxidase with an alternative oxygen ac- ceptor. Annals of Clinical Biochemistry, 1969; 6, 24-27.

Herbert, V, Lau, KS, Gotlieb, CW & Bleicher, ST. Coated charcoals immunoassay of insulin. J Clin Endocrinol Metab. 1965; 25, 1375– 1384.

Habig, W. H; Pabst, M. J and Jacoby, W. B..Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. 1974; J. Biol. Chem., 249:7130-7139.

Paglia, D. E.; valentine, W. N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. 1967; Journal of Laboratory and Clinical Medicine, 70, 1,158-169.

Nishikimi, M., N.A. Rao and K. Yagi. The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem. Biophys. Res. Co. 46: 849–864, 1972.

Aebi, H. Catalase in Vitro. Methods Enzymology, 1984; 105, 121-126.

Arulselvan and S. Subramanian, “Effect of Murraya koenigii leaf extract on carbohydrate metabolism studied in streptozotocin induced diabetic rats,” International Journal of Biological Chemistry, vol. 1, pp. 21–28, 2007.

Baynes and S. R. Thorpe, “Role of oxidative stress in diabetic complications: a new perspective on an old paradigm,” Diabetes, vol. 48, no. 1, pp. 1–9, 1999.

Rathore, M. Kale, S. John, and D. Bhatnagar, “Lipid peroxidation and antioxidant enzymes in isoproterenol induced oxidative stress in rat erythrocytes,” Indian Journal of Physiology and Pharmacology, vol. 44, no. 2, pp. 161–166, 2000.

S¨ozmen, B. S¨ozmen, Y. Delen, and T. Onat, “Catalase/ superoxide dismutase (SOD) and catalase/paraoxonase (PON) ratios may implicate poor glycemic control,” Archives of Medical Research, vol. 32, no. 4, pp. 283–287, 2001.

Sayed, M. Khalifa, and F. F. Abd el-Latif, “Fenugreek attenuation of diabetic nephropathy in alloxan-diabetic rats-attenuation of diabetic nephropathy in rats,” Journal of Physiology and Biochemistry, vol. 68, no. 2, pp. 263–269, 2012.

Lorenzi, “The polyol pathway as a mechanism for diabetic retinopathy: attractive, elusive, and resilient,” Experimental Diabesity Research, vol. 2007, Article ID 61038, 10 pages, 2007.

Prathapan, S. V. Nampoothiri, S. Mini, and K. G. Raghu, “Antioxidant, antiglycation and inhibitory potential of Saraca ashoka flowers against the enzymes linked to type 2 diabetes and LDL oxidation,” European Review forMedical and Pharmacological Sciences, vol. 16, no. 1, pp. 57–65, 2012.


  • There are currently no refbacks.